Coding

Part:BBa_K4258014

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-09-26)


CBDclos-Linker-meffRed-His

Bsa1 sites for Golden Gate cloning as a CD module and Bpi1 sites for cloning into pOGG006 to immortalize the part as a plasmid. Cellulose Binding Domain from Clostridium cellulovorans (BBa_K863111) + 10x GS Linker + MeffRed from BBa_K1033920 + 6x Histidine tag. A coding sequence that will express meffRed (very feint color) with a cellulose binding domain.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 48
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 48
    Illegal NotI site found at 877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 48
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 48
    Illegal NgoMIV site found at 27
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 16
    Illegal BsaI.rc site found at 1082


[edit]
Categories
Parameters
None